Validation of Commercial Formulation of Difenoconazole Using HPLC Equipped with Dad

Sat, 22 Jun 2024 00:00:00 +0100

Effective, selective, precise, and accurate liquid chromatographic analytical methods for the determination of difenoconazole fungicide in the formulation have been optimized and validated

to meet the accreditation requirements, the performance characteristics of the analytical method were validated which is also one of the basic requirements of ISO Standard 17025:2017, for the rapid determination of difenoconazole by HPLC-DAD. The used method involves the extraction of the substances by sonication of the sample with acetonitrile, followed by dilution in acetonitrile, and direct injection on a liquid chromatography system. For the analysis LC system from Agilent Technologies 1290 Infinity II was used. Good separation was achieved on a Luna C18 column (3µm, 100°A, 3x150mm) with a guard column (C18 4x2.0mm I.D), an isocratic mobile elution consisting of acetonitrile and water acidified 0.075% with formic acid (85:15, v/v), at a flow rate of 0.7 ml/minute and UV detection at 220 nm. The column temperature was 25⁰C, injected volume was 1 μL. The analysis duration was 10 minutes (the retention time of difenoconazole and 4 – Hydroxybenzoic acid – methyl ester was 1.846 and 1.135 minutes, respectively. The linearity within the concentration range of 750-125 µg mL^-1, with the internal standard at a concentration of 250 µg mL^-1with an average correlation coefficient (R²) of 0.9998. We have considered precision, repeatability, and selectivity in the validation.

Environmental, Economic and Social Perceptions of Community Members on the Role of Water, Soil and Natural Grasslands as a Basis for Local Development in Acopalca, Peru

R. M Yaranga, E. M Maldonado, S. E Pizarro, D. Cano — Wed, 19 Jun 2024 00:00:00 +0100

The concept of ecosystem services has gained popularity among academics, researchers and policymakers to support environmental management and biodiversity conservation, so that many development projects in rural areas have merited investment for restoration and improvement of grassland ecosystems accompanied by training programs for the beneficiaries, With this criterion in mind, the study investigated the perception of puna pastoralists in Acopalca, Peru, regarding the degree of knowledge about the significance of the ecosystem services provided by soil-water-grasslands, with the objective of characterizing the environmental, social and economic dimensions of this local perception, through the application of a survey to the representative of the livestock family affiliated to two producers' associations. It was evidenced that cattle-raising families have a limited understanding of the role of the natural resources they directly access and little clarity on the relationship between natural pastures, family income and access to basic services. The results revealed limitations in environmental perception, evidencing a lack of knowledge about the multifaceted contribution of pastures. Social perception showed neutrality in the relationship between pastures and family income, and a discrepancy in access to basic services. The comparison between associations highlighted significant differences, indicating the need for training strategies adapted to the local idiosyncrasies of the beneficiaries. In conclusion, addressing the deficiencies identified in community understanding was essential to strengthening sustainable natural resource management in Acopalca. It highlights the importance of designing specific training programs, considering the particularities of each group, to promote self-management and community participation and thus achieve more comprehensive and sustainable local development.

Global Research in Environment and Sustainability

Validation of Commercial Formulation of Difenoconazole Using HPLC Equipped with Dad

Environmental, Economic and Social Perceptions of Community Members on the Role of Water, Soil and Natural Grasslands as a Basis for Local Development in Acopalca, Peru