Genetic Diversity and Fingerprinting of Garcinia indica

Abstract

Genomic DNA of 19 samples from Garcinia indica was isolated.   The average number of alleles across all microsatellite loci stood at 18, whereas, the number of different alleles across microsatellite loci ranged between 6 and 16 with an average of 11.727. (Table1). The microsatellite marker loci ITBTGI19 and ITBTGI21 recorded highest number of different alleles (16). The overall mean of I was 2.296, The grand mean of expected heterozygosity (He) was 0.877. (Table1). Among all the loci. The mean heterozygosity values across all loci ranged between 0.756 (ITBTGI9) to 0.956 (ITBTGI19). The pair-wise genetic distance among the accessions of G. indica_, the values ranged from a minimum of 56 to a maximum of 88 between different accessions the accession GI_MDG_4 recorded genetic distance of 88 with most of the other accessions Table2. The microsatellite ITBTGI1 recorded maximum allelic size of 1000 for the accession GI-KAN 3 (Table3).